Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli
نویسندگان
چکیده
منابع مشابه
Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli.
The Escherichia coli proteins H-NS is recognized as an important component among the major nucleoid-associated proteins. In studies of E. coli strains with defects in H-NS, we discovered a mutant that phenotypically restored stationary-phase viability (Rsv) of such strains. The Rsv phenotype was the result of a mutation that led to severalfold higher levels of the functionally and structurally ...
متن کاملInvolvement of the global regulator H-NS in the survival of Escherichia coli in stationary phase.
Long-term batch cultures of Escherichia coli grown in nutrient-rich medium accumulate mutations that provide a growth advantage in the stationary phase (GASP). We have examined the survivors of prolonged stationary phase to identify loci involved in conferring a growth advantage and show that a mutation in the hns gene causing reduced activity of the global regulator H-NS confers a GASP phenoty...
متن کاملNucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein.
analysis of a 978-bp fragment of this segment revealed an ORF , w . r o , * r. A A , ^ c ,,noo> ^ * o i«> M.-> , J , r . b r IIA • J /i-.N 1Martinez,E., Bartolomi.B. and de la Cruz,F. (1988) Gene 68, 159-162. that encodes a putative protein of 134 ammo acids (Figure 1). 2 DevereuxJ., Haeberli.P. and Smithies.O. (1984) Nucleic Acids Res. 12, Comparative searches (FASTA, TFASTA (2)) showed that t...
متن کاملDifferential dependence of StpA on H-NS in autoregulation of stpA and in regulation of bgl.
StpA has functional similarity to its homologue, the nucleoid structuring protein H-NS. It binds to AT-rich, planar, bent DNA and constrains DNA supercoils. In addition, StpA acts as an RNA chaperone. StpA and H-NS also form heterodimers. However, cellular levels of StpA are low due to repression of stpA by H-NS and negative autoregulation. Here we show that effective (30-fold) repression of st...
متن کاملHeteromeric interactions among nucleoid-associated bacterial proteins: localization of StpA-stabilizing regions in H-NS of Escherichia coli.
The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric H-NS-StpA complexes. The two proteins show differential turnover, and StpA was previously found to be subject to protease-mediated degradation by the Lon protease. We investigated which regions of the H-NS pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1999
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.96.19.10776